Experiment of the effects of enzyme concentration on the activity of salivary amylase

After the animals had been fed xylitol for 14 days the half life fell to 4.

Experiment of the effects of enzyme concentration on the activity of salivary amylase

Wear eye protection when handling iodine solution. Hazards of buffers may vary. It is wise to test, well in advance, the activity of the stored enzyme at its usual working concentration to check that substrates are broken down at an appropriate rate.

Enzymes may degrade in storage and this allows time to adjust concentrations or to obtain fresh stocks. Amylase will slowly lose activity, so it is best to make up a fresh batch for each lesson; batches may vary in activity and results collected on different days will not be comparable.

This has the advantage of being cheaper, not requiring technicians to make up fresh solutions each lesson, it is directly interesting to students, and salivary amylase is reliable. It also provides an opportunity to teach good hygiene precautions — including ensuring that students use only their own saliva samples provide small beakers to spit into ; that students are responsible for rinsing their own equipment; and that all contaminated glassware is placed in a bowl or bucket of sodium chlorate I before technicians wash up.

Make a cream of 5 g soluble starch in cold water. Pour into cm3 of boiling water and stir well. Boil until you have a clear solution. Do not use modified starch. Make this by fold dilution of 0.

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Once made, the solution is a low hazard but may stain skin or clothing if spilled. Ethical issues There are no ethical issues associated with this procedure. All solutions once made up are low hazard. Wear eye protection, as iodine may irritate eyes.

Preparation a Check the speed of the reaction with the suggested volumes of reactants to be used — 2 cm3 of starch: Ideally the reaction should take about 60 seconds at this pH: If the reaction is too fast, either reduce the enzyme volume or increase the starch volume.

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If the reaction is too slow, increase the enzyme volume or concentration or reduce the starch volume or concentration. Investigation b Place single drops of iodine solution in rows on the tile. Mix using a plastic pipette.

The iodine solution should turn blue-black. If the iodine solution remains orange the reaction is going too fast and the starch has already been broken down. Squirt the rest of the solution in the pipette back into the test tube. Then remove a second drop of the mixture to add to the next drop of iodine.

What should this contain? Teaching notes This is a straightforward practical giving reliable, unambiguous results. Temperature variation affects enzyme activity, so results collected on different days are not comparable. Health and safety checked, September Downloads.I n this post, I summarize the literature I could gather on dextrins, focusing the research primarily on how dextrins may influence mouthfeel and brewing conditions that may favor dextrins.

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Experiment of the effects of enzyme concentration on the activity of salivary amylase

Note: Most projects are for more than one grade and selection depends on your previous knowledge about the subject. Do not select projects that you are not familiar with. Introduction. Amylase is an enzyme present in saliva and pancreatic juice. It catalyses the hydrolysis of amylose and amylopectin (both starch components) to a mixture of products including maltose and dextrin..

Aim. To investigate the effect of amylase concentration on its activity. The relative activity is determined by noting the time taken for the starch substrate to break down.

PEKA experiment list for SPM Biology Form 4 Chapter 6